Suetsuna, K.; Nagatomo, K.; Doi, K.
JOURNAL NAME- Journal of Shimonoseki University of Fisheries
VOL. 42
NO. 4
1994
PP. 171-183
ISSN- 0370-9361
AUTHOR AFFILIATION- Lab. Food Eng. Processing, Dep. Food Sci. Technol., Shimonoseki Univ. Fisheries, Shimonoseki, Japan
LITERARY INDICATOR(S)- RESEARCH ARTICLE
PRINT PRODUCT NUMBER- Biological Abstracts Vol. 099 Iss. 001 Ref. 010398
LANGUAGE- English NDN- 007-0472-0479-9
A fraction of alpha-amylase inhibitor was extracted from the mycelia of a marine Actinomycetes, Streptomyces sp. No.2020 found in seawater. The fraction was purified using activated charcoal and Dowex 50W column chromatography. The basic structure of the fraction of alpha-amylase inhibitor (AI-2020) was identified as oxireno (e) pyrrolo (2, 1-b) benzoxazole-2, 3, 5, 6-tetraol, decahydro-7- (1-hydroxyethyl) 1a- (hydroxymethyl) -, structure formula 11, on the basis of the elementary analysis, mass spectrum, infrared (IR) and nuclear magnetic resonance (NMR) spectra of the hydrolyzate of AI-2020. The AI-2020 suppressed the elevation of blood glucose in vivo after oral administration of starch. In streptozotocin diabetic rats receiving AI-2020, triglyceride, free fatty acid, total cholesterol and beta-lipoprotein were reduced, and AI-2020 ameliorated diabetes syndrome including symptoms such as hyperglycemia. These findings indicate that AI-2020 may be useful for control of some carbohydrate-dependent diseases such as diabetes, obesity and hyperlipemia.